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tgfβ  (R&D Systems)


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    Structured Review

    R&D Systems tgfβ
    Tgfβ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf%CE%B2+1/Recombinant+Human+TGF-beta+1+Protein/pm41932341-861-54-55
    Average 97 stars, based on 1993 article reviews
    tgfβ - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Convergent and divergent immune aberrations in COVID-19, post-COVID-19-Interstitial Lung Disease and Idiopathic Pulmonary Fibrosis
    Article Snippet: .. We measured cytokine concentrations of 63 plasma samples from COVID-19 patients from Cohort 1 using a customized, Bioplex 200 compatible, human cytokine panel including, IL6, IP10, SPP1 and TGFβ−1 (#FCSTM18–06, R&D Systems). ..

    Article Title: Convergent and divergent immune aberrations in COVID-19, post-COVID-19-Interstitial Lung Disease and Idiopathic Pulmonary Fibrosis.
    Article Snippet: 32 We aimed to study transcriptional and phenotypic changes in circulating immune cells associated 33 with increased risk of mortality in COVID-19, resolution of pulmonary fibrosis in post-COVID34 19-Interstitial Lung Disease (ILD) and persistence of Idiopathic Pulmonary Fibrosis.. Whole 35 blood and Peripheral Blood Mononuclear cells (PBMC) were obtained from 227 subjects with 36 COVID-19, post-COVID-19 Interstitial Lung Disease (ILD), IPF and controls.. We measured a 37 50-gene signature (nCounter, Nanostring) previously found to be predictive of IPF and COVID38 19 mortality along with plasma levels of several biomarkers by Luminex.

    Article Title: A 50-gene high-risk profile predictive of COVID-19 and Idiopathic Pulmonary Fibrosis mortality originates from a genomic imbalance in monocyte and T-cell subsets that reverses in survivors with post-COVID-19 Interstitial Lung Disease
    Article Snippet: .. We measured cytokine concentrations of 121 plasma samples from COVID-19 patients from Cohort 1 using a customized, Bioplex 200 compatible, human cytokine panel including, IL6, IP10, SPP1 and TGFβ-1 (#FCSTM18-06, R&D Systems). ..

    Neutralization:

    Article Title: Visceral fat-specific regulation of plasminogen activator inhibitor-1 in aged septic mice.
    Article Snippet: Aging and Critical Care Research Laboratory, University of Kentucky, Lexington, Kentucky, USA Department of Surgery, University of Kentucky, Lexington, Kentucky, USA Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, Kentucky, USA Department of Statistics, University of Kentucky, Lexington, Kentucky, USA Department of Physiology, University of Kentucky, Lexington, Kentucky, USA

    Transfection:

    Article Title: SMAD3 mutation in LDS3 causes bone fragility by impairing the TGF-β pathway and enhancing osteoclastogenesis
    Article Snippet: The following day, cells were transfected for 24 h with X-tremeGENETM HP DNA Transfection Reagent (6,366,236,001, Sigma-Aldrich) and 1 μg/well plasmid DNA constructs expressing pcDNA3.1/NT-GFP-TOPOTM (K481001, NT-GFP Fusion TOPOTM Expression Kit, InvitrogenTM), CS2 Flag-SMAD3 (14052, Addgene), and mutant CS2 Flag-SMAD3 (p.I67S). .. After transfection, cells were stimulated with 5 ng/mL of TGFβ-1 (7754-BH, R&D) for another 24 h and harvested for RNA. .. Cultured and treated cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (ThermoFisher Scientific, Waltham, MA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Serglycin controls megakaryocyte retention of platelet factor 4 and influences megakaryocyte fate in bone marrow
    Article Snippet: .. Additionally, TGFβ-1, MIP-1α, and Osteopontin (R&D systems: DY1679, DY450, and DY441 respecitvely) concentrations were assessed by ELISA following manufacturers protocols. ..

    Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice.
    Article Snippet: .. To activate splenic lymphocytes, 2.5 × 106 cells were seeded in 24-well plates and stimulated with 500 ng/ml of Lipopolysaccharide (LPS, from Sigma-Aldrich, St Louis, MO, United States) or anti-mouse CD3ε (plate coated at 5 μg/ml, Bio X cell, Lebanon, NH, United States) plus 2 μg/ml soluble antimouse CD28 (Bio X cell) for 24 h h, or 50 ng/ml phorbol 12- myristate 13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) for 6 h. For the induction of IL-17, the splenocytes were stimulated with either non-pathogenic T helper 17 (Th17) stimuli (20 ng/ml IL-6 (BioLegend) plus 3 ng/ml TGFβ 1 (R&D System Inc., Minneapolis, MN, United States) and 1 μg/ml anti-CD3) or pathogenic Th17 stimuli (20 ng/ml IL-6 plus 10 ng/ml IL-1β (BioLegend), 10 ng/ml IL-23 (Invitrogen) and 1 μg/ml anti-CD3) for 72 h. The cell culture supernatants were collected for ELISA assay. ..

    Cell Culture:

    Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice.
    Article Snippet: .. To activate splenic lymphocytes, 2.5 × 106 cells were seeded in 24-well plates and stimulated with 500 ng/ml of Lipopolysaccharide (LPS, from Sigma-Aldrich, St Louis, MO, United States) or anti-mouse CD3ε (plate coated at 5 μg/ml, Bio X cell, Lebanon, NH, United States) plus 2 μg/ml soluble antimouse CD28 (Bio X cell) for 24 h h, or 50 ng/ml phorbol 12- myristate 13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) for 6 h. For the induction of IL-17, the splenocytes were stimulated with either non-pathogenic T helper 17 (Th17) stimuli (20 ng/ml IL-6 (BioLegend) plus 3 ng/ml TGFβ 1 (R&D System Inc., Minneapolis, MN, United States) and 1 μg/ml anti-CD3) or pathogenic Th17 stimuli (20 ng/ml IL-6 plus 10 ng/ml IL-1β (BioLegend), 10 ng/ml IL-23 (Invitrogen) and 1 μg/ml anti-CD3) for 72 h. The cell culture supernatants were collected for ELISA assay. ..



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